stat3 phosphorylation inhibitor stattic  (MedChemExpress)

Journal: Brain Sciences

Article Title: Minocycline Ameliorates Staphylococcus aureus -Induced Neuroinflammation and Anxiety-like Behaviors by Regulating the TLR2 and STAT3 Pathways in Microglia

doi: 10.3390/brainsci15020128

Figure Lengend Snippet: Effects of minocycline on STAT3 activation and GLS1 expression in LTA-induced microglial cells. ( A , B ) The expression levels of p-STAT3, STAT3, and GLS1 in the BV2 and N9 cells were analyzed via Western blot analysis. ( C , D ) The quantification of the expression levels of p-STAT3/STAT3 and GLS1 in BV2 and N9 cells. The data were normalized according to GAPDH. The mean ± SEM of three separate experiments is represented for all data that have error bars. Compared to the control group, * p < 0.05; ** p < 0.01; *** p < 0.001. Compared to the LTA group, # p < 0.05; ## p < 0.01; ### p < 0.001. Control, saline-treated group; LTA, cells pretreated with saline and then stimulated with LTA; Mino100 + LTA, cells pretreated with 100 μmol/L minocycline and then stimulated with LTA; Mino200 + LTA, cells pretreated with 200 μmol/L minocycline and then stimulated with LTA.

Article Snippet: The STAT3 phosphorylation (at Y705 and S727) inhibitor Stattic was purchased from MCE (Princeton, NJ, USA).

Techniques: Activation Assay, Expressing, Western Blot, Control, Saline

Journal: Brain Sciences

Article Title: Minocycline Ameliorates Staphylococcus aureus -Induced Neuroinflammation and Anxiety-like Behaviors by Regulating the TLR2 and STAT3 Pathways in Microglia

doi: 10.3390/brainsci15020128

Figure Lengend Snippet: The inhibition of p-STAT3 reduces the proinflammatory cytokines and GLS1 levels in LTA-induced microglial cells. ( A , B ) The TNF-α, IL-6, and IL-10 secreted by the BV2 and N9 cells were assessed via ELISA. ( C , D ) The expression levels of TLR2, TNF-α, IL-6, GLS1, p-STAT3, and STAT3 in the BV2 and N9 cells were analyzed via Western blot analysis. ( E , F ) The quantification of the expression levels of p-STAT3/STAT3, TLR2, TNF-α, IL-6, and GLS1 in the BV2 and N9 cells. The data were normalized according to GAPDH. The mean ± SEM of three separate experiments is represented for all data that have error bars. Compared to the control group, * p < 0.05; ** p < 0.01; *** p < 0.001. Compared to the LTA group, # p < 0.05; ## p < 0.01; ### p < 0.001. Control, saline-treated group; LTA, cells pretreated with saline and then stimulated with LTA; Stattic, cells pretreated with 10 μmol/L Stattic and then treated with saline; Stattic + LTA, cells pretreated with 10 μmol/L Stattic and then stimulated with LTA.

Article Snippet: The STAT3 phosphorylation (at Y705 and S727) inhibitor Stattic was purchased from MCE (Princeton, NJ, USA).

Techniques: Inhibition, Enzyme-linked Immunosorbent Assay, Expressing, Western Blot, Control, Saline

Journal: Brain Sciences

Article Title: Minocycline Ameliorates Staphylococcus aureus -Induced Neuroinflammation and Anxiety-like Behaviors by Regulating the TLR2 and STAT3 Pathways in Microglia

doi: 10.3390/brainsci15020128

Figure Lengend Snippet: Effects of minocycline on S. aureus -induced neuroinflammation in vivo. ( A ) The GLS1, p-STAT3, and STAT3 expression levels in the mPFC of two representative mice were analyzed via Western blot analysis. ( B ) The expression levels of p-STAT3/STAT3 and GLS1 in the mPFC were quantified. The data were normalized according to GAPDH. Data are presented as mean ± SEM, n = 4. ( C ) The TLR2, TNF-α, and IL-6 protein expression levels in the mPFC of two representative mice were analyzed via Western blot analysis. ( D ) The expression levels of TLR2, TNF-α, and IL-6 in the mPFC were quantified. The data were normalized according to GAPDH. Data are presented as mean ± SEM, n = 4. ( E ) Schematic diagram showing the mPFC area (red box) of the mice analyzed via immunofluorescent staining. ( F ) Representative fluorescence micrographs showing the number of microglia in the mPFC in the various groups (IBA1, red; DAPI, blue); scale bar = 100 μm. ( G ) A quantitative analysis of the number of IBA1 + cells in the mPFC. Data are presented as mean ± SEM, n = 3. USA300 group compared to the control group, * p < 0.05. Mino + USA300 group compared to the USA300 group, # p < 0.05; ## p < 0.01. Control, saline-treated group; Mino, mice pretreated with minocycline and then challenged with the saline group; USA300, mice pretreated with saline and then challenged with the USA300 group; Mino + USA300, mice pretreated with minocycline and then challenged with the USA300 group.

Article Snippet: The STAT3 phosphorylation (at Y705 and S727) inhibitor Stattic was purchased from MCE (Princeton, NJ, USA).

Techniques: In Vivo, Expressing, Western Blot, Staining, Fluorescence, Control, Saline

Journal: Translational Cancer Research

Article Title: Tumor-treating fields in combination with sorafenib curtails the growth of colorectal carcinoma by inactivating AKT/STAT3 signaling

doi: 10.21037/tcr-21-1853

Figure Lengend Snippet: TTFields combined sorafenib inhibits the phosphorylation of STAT3 and AKT and then suppresses STAT3 transcription activity. (A) Western blot analysis of HCT116 cell lysates, following 24 h cell culture in the presence of LY294002 (10 µM) or Stattic (10 µM). (B) Prior to being treated for 36 hours before dual-luciferase assay, SW480 and HCT116 cells were transfected with internal control pRL-SV40 Renilla luciferase construct and nonresponsive control reporter pLucTK/pLucTKS3. **P<0.01. TTF, tumor-treating fields; S + T, sorafenib + tumor-treating fields; S, sorafenib; TTFields, tumor-treating fields.

Article Snippet: Stattic hydrochloride (>95%) which is a potent STAT3 inhibitor and inhibits STAT3 phosphorylation (at Y705 and S727) was purchased from Sigma-Aldrich (19983-44-9).

Techniques: Activity Assay, Western Blot, Cell Culture, Luciferase, Transfection, Control, Construct